Characterization of a 50-kDa Polypeptide in Cytoplasmic Dynein Preparations Reveals a Complex with p150GLUED
نویسندگان
چکیده
From the $Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545, the TDepartment of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, the 11 Department of Anatomy and Cell Biology, University of Virginia Health Sciences Center, Charlottesuille, Virginia 22908, and the **Department of Biological Chemistry, School of Medicine, University of California, Los Angeles, California 90024
منابع مشابه
Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued
Cytoplasmic dynein is a retrograde microtubule motor thought to participate in organelle transport and some aspects of minus end-directed chromosome movement. The mechanism of binding to organelles and kinetochores is unknown. Based on homology with the Chlamydomonas flagellar outer arm dynein intermediate chains (ICs), we proposed a role for the cytoplasmic dynein ICs in linking the motor prot...
متن کاملThe involvement of the intermediate chain of cytoplasmic dynein in binding the motor complex to membranous organelles of Xenopus oocytes.
Cytoplasmic dynein is one of the major motor proteins involved in intracellular transport. It is a protein complex consisting of four subunit classes: heavy chains, intermediate chains (ICs), light intermediate chains, and light chains. In a previous study, we had generated new monoclonal antibodies to the ICs and mapped the ICs to the base of the motor. Because the ICs have been implicated in ...
متن کاملA requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization
We present evidence that vimentin intermediate filament (IF) motility in vivo is associated with cytoplasmic dynein. Immunofluorescence reveals that subunits of dynein and dynactin are associated with all structural forms of vimentin in baby hamster kidney-21 cells. This relationship is also supported by the presence of numerous components of dynein and dynactin in IF-enriched cytoskeletal prep...
متن کاملA cytoplasmic dynein motor in Drosophila: identification and localization during embryogenesis.
We have characterized a cytoplasmic dynein motor isoform that is present in extracts of Drosophila embryos. A prominent high molecular weight (HMW) polypeptide (> 400 kDa) is enriched in microtubules prepared from nucleotide-depleted embryonic extracts. Based on its ATP-sensitive microtubule binding activity, 20 S sedimentation coefficient, sensitivity to UV-vanadate and nucleotide specificity,...
متن کاملMolecular analysis of a cytoplasmic dynein light intermediate chain reveals homology to a family of ATPases.
Cytoplasmic dynein is a multi-subunit complex involved in retrograde organelle transport and some aspects of mitosis. In previous work we have cloned and sequenced cDNAs encoding the rat cytoplasmic dynein heavy and intermediate chains. Here we report the cloning of the remaining class of cytoplasmic dynein subunits, which we refer to as the light intermediate chains (LICs: 53-59 kDa). Four LIC...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2001